Voltage-dependent Ca 2+ currents in rat cardiac dorsal root ganglion neurons

Abstract

This study presents the kinetic and pharmacological properties of voltage-gated Ca 2+ currents in anatomically defined cardiac dorsal root ganglion (DRG) neurons in rats. The neurons were labelled by prior injection of fluorescent tracer Fast Blue into the pericardial sack. There were three distinct groups of neurons with respect to cell size: small (27% of total; cell capacitance <30 pF), medium (65% of total; capacitance 30–80 pF) and large neurons (8% of total; capacitance >80 pF). The properties of Ca 2+ currents were tested in small and medium-sized neurons. In large neurons currents could not be adequately controlled and were not analysed. Ca 2+ currents did not completely inactivate during 100 ms depolarising voltage steps. The activation thresholds in small (−36.9±1.3 mV) and medium (−39.0±1.3 mV) size neurons were similar. Current densities were 105.8 pA/pF in small and 97.4 pA/pF in large neurons and also did not differ. The kinetic properties of activation and inactivation did not differ between small and medium-sized cardiac DRG neurons. At membrane potentials between −50 and −60 mV (the expected resting membrane potential in these neurons) 55 to 70% of Ca 2+ currents in small and medium-sized neurons were available for activation. Both, small and medium-sized neurons expressed similar proportions of L (7.5%), N (25%) and P/Q (36%) type Ca 2+ currents. We conclude that small and medium-sized cardiac DRG neurons are homogeneous with respect to the expression and properties of voltage-gated Ca 2+ currents. Voltage-gated Ca 2+ currents probably play an important role in action potential generation in cardiac DRG neurons due to their availability for activation at resting membrane potential, their high density and voltage threshold close to the threshold for voltage-gated Na + currents.