Vitamin E and C Long-Term Treatment Effect on paraquat Poisoned Liver in Rat

Abstract

Paraquat toxicant causes serious herbicide intoxication generally and typically because of intentional or unintentional ingestion. Vitamin C and E are awesome antioxidants that respond quickly to oxidative stress and modify lipid peroxidation. This study was aimed at assessing the impact of Vitamin E and C combined treatment on chronic toxicity of paraquat on liver organs in Wistar rats. Exactly 200 male rats were used for the study. The rodents were isolated into four groups of 50 rats in each group (A, B, C, D) and was further subgrouped into two (0 and VEC), having 25rats per subgroup. Each of the "0" subgroups (A0, B0, C0 and D0) aside from A0 were treated with paraquat in 0.02g, 0.04g and 0.06g respectively for 3 months every 2 weeks. All "VEC" subgroups (AVEC, BVEC, CVEC and DVEC) aside from AVEC were treated with paraquat also, after which they were treated with vitamin E (500mg) and C (2000mg/L) week after week for three months. AVEC was treated with vitamin E and C alone. Blood samples were obtained and tested for liver function (total, conjugated, unconjugated bilirubin, total protein, albumin and globulin). There was a significant difference (p-value<0.05) in the level of the liver parameters among the "Ao", "Bo", "Co" and "Do". The outcome additionally showed that there were significant difference (p-value<0.05) in the levels of the liver parameters within the groups (Bo vs BVEC, Co vs CVEC, and Do vs DVEC). However, was no significant difference in the levels of globulin among the subgroups. This study has affirmed that vitamin E and C combined therapy has a helpful impact in male Wistar rats on week after week treatment for 3 months.