Abstract
Background: Lumbar disc degeneration [LDD] is the main cause of low back pain, and is a medical circumstance that poses a great load on the global health care system with serious socioeconomic consequences. The aim of this study was to determine the association of vitamin D receptors (VDR) gene Fok1 polymorphism, VDR expression and vitamin D level with LDD susceptibility and severity in Egyptians. Materials and methods: A total of unrelated 169 female patients with low back pain diagnosed as having lumbar disc degeneration and 169 controls were enrolled in the study. VDR Fok1 polymorphism was determined using restriction fragment length polymorphism-polymerase chain reaction [PCR-RFLP]. VDR expression was measured by reverse transcriptase PCR, while, serum levels of vitamin D were measured by high performance liquid chromatography [HPLC]. Results: Subjects with VDR Fok1 ff genotype were significantly more likely to develop LDD in Egyptians and associated with multilevel disc degeneration and disc herniation (adjusted OR = 7.9, 95% CI = 2.3-26.6 and P = 0.01) and (adjusted OR = 2.2, 95% CI = 1.6-3.7 and P = 0.031) respectively. VDR expression and vitamin D levels were significantly decreased in LDD patients and with multilevel disc degeneration and herniation, (adjusted P = 0.042 and adjusted P = 0.028) respectively. Conclusion: VDR Fok1 mutant alleles and genotypes are associated with the development and severity of LDD in Egyptians. Also, VDR expression and vitamin D level could be the marker for monitoring LDD.
Highlights
Materials and methods: A total of unrelated 169 female patients with low back pain diagnosed as having lumbar disc degeneration and 169 controls were enrolled in the study
On the foundation of this consideration, we proposed to investigate whether vitamin D receptors (VDR) Fok1 polymorphism, VDR expression and vitamin D level contributed to Lumbar disc degeneration (LDD) susceptibility and severity of in Egyptians
We observed significant increase of VDR Fok1ff genotype and f allele frequencies in LDD patients compared to control group
Summary
Lumbar disc degeneration [LDD] is the main cause of low back pain, and is a medical circumstance that poses a great load on the global health care system with serious socioeconomic consequences. The aim of this study was to determine the association of vitamin D receptors (VDR) gene Fok polymorphism, VDR expression and vitamin D level with LDD susceptibility and severity in Egyptians. Results: Subjects with VDR Fok ff genotype were significantly more likely to develop LDD in Egyptians and associated with multilevel disc degeneration and disc herniation (adjusted OR = 7.9, 95% CI = 2.3 − 26.6 and P = 0.01) and (adjusted OR = 2.2, 95% CI = 1.6 − 3.7 and P = 0.031) respectively. VDR expression and vitamin D levels were significantly decreased in LDD patients and with multilevel disc degeneration and herniation, (adjusted P = 0.042 and adjusted P = 0.028) respectively. VDR expression and vitamin D level could be the marker for monitoring LDD
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