Abstract
Limb bud cells were isolated from HH stage 22–23 chick embryos and were grown as a ‘spot culture’ in in vitro conditions which support their differentiation into chondrocytes and myotubes. By day 4 of culture, numerous chondrocyte nodules developed and were scattered mainly in the very centre of the cell spot. In contrast, multinucleated myotubes formed at both the centre and the periphery of the cell spot. Treatment with vitamin A starting on day 1, inhibited chondrogenesis in these cultures, and by day 4–6 chondrocyte nodules could not be detected histologically. In contrast, no dose of vitamin A tested was effective in suppressing the development of multi-nucleated myotubes. These data show that vitamin A selectively inhibits chondrogenesis but not myogenesis in limb bud cell cultures.
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