Visualizing peroxynitrite fluxes and protective effect of endogenous hydrogen sulfide during carbonyl stress in endothelial cell

Abstract

Carbonyl stress is caused by active carbonyl compounds. Abnormal metabolism will produce high concentration of active carbonyl substances, leading to carbonyl stress, thus inducing cell damage or apoptosis. It is important to find effective markers for carbonyl stress level and find therapeutic or palliative drugs. Peroxynitrite (ONOO−), as a kind of important reactive oxygen species, can be produced under a variety of pathological conditions. Hydrogen sulfide (H2S) has been known as antioxidant agent, anti-inflammatory et al. However, the protective effect of H2S and the level of ONOO− in endothelial cell apoptosis during carbonyl stress kept unknown. Herein, for address it, we reported the first single-molecule fluorescent probe (SZ) is capable of detecting ONOO− and H2S in Endothelial cell. The probe SZ shows good selectivity and low detection limit to ONOO− and H2S. More importantly, probe SZ may be a useful tool for evaluating and confirming the protective effects of endogenous H2S in Endothelial cell during carbonyl stress.