Abstract
Alzheimer’s disease (AD) is now clinically considered as a chronic inflammation-based neurodegenerative disease. The CDnir7 probe was previously developed as an optical imaging probe to target macrophages in order to image mouse inflammation using in vivo optical imaging modalities such as In Vivo imaging system (IVIS) and fluorescent molecular tomography (FMT). Here, we demonstrate the application of CDnir7 in AD mouse brain imaging via multispectral optoacoustic tomography (MSOT). Longitudinal MSOT imaging of CDnir7 showed higher CDnir7 localization in AD mouse cerebral cortex compared to that of normal mice. MSOT signals of CDnir7 localization in mouse brain were verified by ex vivo near-infrared (NIR) imaging and immunohistochemistry. Histological evaluation showed strong CDnir7 staining in AD cerebral cortex, hippocampus, basal ganglia and thalamus area. Based on the supporting evidence, CDnir7 has great potential as a molecular imaging probe for AD brain imaging.
Highlights
We demonstrate that, in the context of Alzheimer’s disease (AD), this CDnir[7] probe can be used in multispectral optoacoustic tomography (MSOT) imaging to distinguish AD mouse brains from healthy brains
13- and 15-month-old triple-transgenic AD mice (n = 5) were utilized for MSOT imaging since AD symptoms can be readily studied via histopathology from 12 months of age onwards
We evaluated the application of an optical imaging probe, CDnir[7], for differentiating AD brains from healthy controls via MSOT imaging and histopathology
Summary
We demonstrate that, in the context of AD, this CDnir[7] probe can be used in MSOT imaging to distinguish AD mouse brains from healthy brains. We examined the CDnir[7] staining pattern in AD mouse brain by processing sectioned brain samples using histological ex vivo methods such as NIR scanning and immunohistochemistry (IHC).
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