Viscosity Measurement of Biological Fluids Using Optical Tweezer

Abstract

An optical tweezer (OT) has been widely used to study the mechanical properties of microscopic living biological systems like red blood cells. These studies are based on measurement of deformations caused by a force exerted directly or indirectly by an optical trap. The trap is usually pre-calibrated using Stokes viscous force of the suspension fluids for the biological system which is directly proportional to the viscosity of the fluids. Therefore, calibration of the trap depends on the viscosity of the fluid which depends on temperature. In this work, we have demonstrated that OT can be used to precisely measure the viscosity of biological fluids affected by temperature. Using a an infrared laser trap which is calibrated using a 3.1micron silica sphere suspended in a distilled deionized water and measuring the power as function of escape velocity, we have measured the viscosities of a newborn and unborn bovine serum with a different concentration of antibodies. Comparative analysis of these measurements with the measurements carried out by direct use of a viscometer have revealed a significant effect of increase in temperature resulting from the intense beam of the laser trap.