VIOLATION OF THE REDOX BALANCE IN NON-MALIGNANT CELLS OF PRIMARY ONCOGYNECOLOGICAL PATIENTS

Abstract

Summary. Oncogynecological diseases in women are one of the global health problems, both in Ukraine and worldwide. An integral part of the treatment of such patients is radiation therapy, the consequence of which is the development of early and distant side complications from the tissues and organs surrounding the irradiated tumor. The initial link in the implementation of radiation complications is a violation of the redox balance that precedes the formation of genetic instability of cells. Aim: to investigate the changes in indicators of the redox balance in the blood of oncogynecological patients before the start of antitumor therapy as prognostic markers of radiation damage. Object and methods: the study was conducted on peripheral blood samples from 30 patients with cervical cancer (CC) and 38 patients with endometrial cancer (EC) as well as 33 conditionally healthy individuals. Patients received treatment at the National Cancer Institute of the Ministry of Health of Ukraine. The intensity of superoxide anion radical (O2–•) generation by peripheral blood lymphocytes (PBL) and pro-antioxidant ratio (PAR) in hemolysate were determined by chemiluminescence-induced methods and malondialdehyde (MDA) content in blood plasma by spectrophotometric method. Results: the analysis of the study results of the indicators ofredox balance in the blood of oncogynecological patients established that non-malignant cells surrounding the tumor, even before the start of antitumor therapy, are functionally altered. A significant increase in the intensity of O2 •- generation in PBL by 1.69 and 1.53 times, a 2.73 and 3.22-fold increase in the MDA content in the blood plasma, and a 1.37 and 1.34-fold increase in PAR in the hemolysate of patients with CC and EC, respectively, was revealed. This indicates an enhancement of prooxidant processes and, therefore, a decrease in the body’s antioxidant defense. A wide range of values for the studied parameters was registered, indicating the need for a personalized approach to planning and conducting radiation therapy for cancer patients. Conclusions: the obtained data indicate a violation of the redox balance in non-malignant cells surrounding the tumor in primary oncogynecological patients, which is a prerequisite for the development of early and distant radiation complications after anticancer treatment. The results provide a basis to considerit expedient to determine the studied parameters before the start of the course of antitumor therapy as prognostic biomarkers of radiation to predict the severity of healthy tissue damage and the personalization of treatment measures for oncogynecological patients.