Abstract
Dynamical properties of functional groups in 2�-deoxyadenosine-5�-monophosphate (dAMP) compound, were identified by UV resonance Raman spectroscopy (UVRR), upon varying nucleotide concentration in aqueous solution (200-600 μM). The studied full-widths at half-maximum (fwhm�s) were found between 13 - 21 cm-1 and the corresponding global relaxation times were faster than 0.817 ps and slower than 0.506 ps. Also, the band around 1430 cm-1 (C4N9-δC8H) in the UV resonance Raman spectrum of dAMP molecule at 400 μM concentration in aqueous solution, was selected for vibrational band shape analysis through time correlation function (CF) concept. Current theories developed for vibrational dephasing (Kubo-Rothschild and Oxtoby) have been applied to this profile and relevant relaxation parameters have been obtained and discussed. The best fit parameters for this dissipation channel of the vibrational excitation energy were established. To our knowledge this is the first UVRR study on nucleotide vibrational band shape analysis through time correlation function concept.
Highlights
Dynamical properties of functional groups in 2’-deoxyadenosine-5’-monophosphate compound, were identified by UV resonance Raman spectroscopy (UVRR), upon varying nucleotide concentration in aqueous solution (200-600 μM)
We report an UV resonance Raman spectroscopic study on the subpicosecond dynamics of functional groups in 2’-deoxyadenosine-5’-monophosphate in aqueous solutions, at different concentrations
DAMP concentration-dependences of full-widths at half-maximum and global relaxation times The selection of the concentration in the interval 2·10-4 - 6·10-4 mol· dm-3 is depending on the hypothesis to work with an isolated molecule, i. e. the relaxation processes are related to an isolated molecule present in the aqueous medium
Summary
Dynamical properties of functional groups in 2’-deoxyadenosine-5’-monophosphate (dAMP) compound, were identified by UV resonance Raman spectroscopy (UVRR), upon varying nucleotide concentration in aqueous solution (200-600 μM). The band around 1430 cm-1 (C4N9-δC8H) in the UV resonance Raman spectrum of dAMP molecule at 400 μM concentration in aqueous solution, was selected for vibrational band shape analysis through time correlation function (CF) concept.
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