Abstract
Parkinson’s disease (PD) is the second most common neurodegenerative disease in the United States after Alzheimer’s disease (AD). To help understand the electrophysiology of these diseases, N27 neuronal cells have been used as an in vitro model. In this study, a flexible graphene-based biosensor design is presented. Biocompatible graphene was manufactured using a liquid-phase exfoliation method and bovine serum albumin (BSA) for further exfoliation. Raman spectroscopy results indicated that the graphene produced was indeed few-layer graphene (FLG) with 0.11. Inkjet printing of this few-layer graphene ink onto Kapton polyimide (PI) followed by characterization via scanning electron microscopy (SEM) showed an average width of ≈868 µm with a normal thickness of ≈5.20 µm. Neuronal cells were placed on a thermally annealed 3D printed graphene chip. A live–dead cell assay was performed to prove the biosensor biocompatibility. A cell viability of approximately 80% was observed over 96 h, which indicates that annealed graphene on Kapton PI substrate could be used as a neuronal cell biosensor. This research will help us move forward with the study of N27 cell electrophysiology and electrical signaling.
Highlights
Parkinson’s disease (PD) is a neuronal disease that is caused by the death of dopaminergic neurons in the substantia nigra pars compacta region of the brain [1]
A cell viability of approximately 80% was observed over 96 h, which indicates that annealed graphene on Kapton PI substrate could be used as a neuronal cell biosensor
Since a study on the electrophysiology of N27 cells is necessary to further understand PD and other neurodegenerative diseases, graphene may be helpful to better understand neurodegenerative diseases when employed as a biosensor
Summary
Parkinson’s disease (PD) is a neuronal disease that is caused by the death of dopaminergic neurons in the substantia nigra pars compacta region of the brain [1] It is currently the second most common neurodegenerative disease in the United States after Alzheimer’s disease (AD) [1,2]. Because of their dopaminergic properties [1], rat dopaminergic N27 cells have been widely used in in vitro models for PD studies [1,3]. Since a study on the electrophysiology of N27 cells is necessary to further understand PD and other neurodegenerative diseases, graphene may be helpful to better understand neurodegenerative diseases when employed as a biosensor
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