Abstract
Instrumental factors influencing very-high-speed liquid chromatography including injection volume, detector flowcell volume, detector response time and total instrumental bandwidth are discussed. Very-high-speed analyses performed using relatively short columns (100mm) of conventional internal diameter (4.6 mm) packed with small particles (3 μm) result in very high performance as determined by the ability to generate over 400 theoretical plates/sec. Minimizing the total instrumental bandwidth by reducing volumes of the injector, connecting tubes and detector flowcell is necessary along with a very rapid detector response time in order to attain this performance. Limitations in performance on peaks having low capacity factors are apparent due to extra-column effects. The relatively high flow-rates which are utilized result in unexpectedly improved chromatographic performance since extra-column effects due to the flow path are not as great at higher flow-rates as originally expected. The largest contribution to extra-column effects appears to arise from the detector flowcell volume and the detector response time.
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