Abstract
Strains of a novel anaerobic, Gram-negative coccus were isolated from the supra-gingival plaque of children. Independent strains from each of six subjects were shown, at a phenotypic level and based on 16S rRNA gene sequencing, to be members of the genus Veillonella. Analysis revealed that the six strains shared 99.7 % similarity in their 16S rRNA gene sequences and 99.0 % similarity in their rpoB gene sequences. The six novel strains formed a distinct group and could be clearly separated from recognized species of the genus Veillonella of human or animal origin. The novel strains exhibited 98 and 91 % similarity to partial 16S rRNA and rpoB gene sequences of Veillonella parvula ATCC 10790T, the most closely related member of the genus. The six novel strains could be differentiated from recognized species of the genus Veillonella based on partial 16S rRNA and rpoB gene sequencing. The six novel strains are thus considered to represent a single novel species of the genus Veillonella, for which the name Veillonella rogosae sp. nov. is proposed. The type strain is CF100T (=CCUG 54233T=DSM 18960T).
Highlights
65093 G 2008 IUMS Printed in Great Britain discriminating phenotypic tests (Kolenbrander & Moore, 1992)
Very low levels of 16S rRNA gene sequence dissimilarity are found within the genus and, V. atypica, V. montpellierensis and the species isolated from rodents are identifiable by this means, discrimination between V. dispar, V. parvula and V. denticariosi is problematic (Jumas-Bilak et al, 2004; Byun et al, 2007)
All recognized members of the genus can be discriminated by using dnaK gene sequence comparison and, in particular, V. parvula and V. dispar have been reliably identified in this way (JumasBilak et al, 2004)
Summary
65093 G 2008 IUMS Printed in Great Britain discriminating phenotypic tests (Kolenbrander & Moore, 1992). Analysis of 16S rRNA gene sequences permits the identification of isolates as members of the genus Veillonella. Very low levels of 16S rRNA gene sequence dissimilarity are found within the genus and, V. atypica, V. montpellierensis and the species isolated from rodents are identifiable by this means, discrimination between V. dispar, V. parvula and V. denticariosi is problematic (Jumas-Bilak et al, 2004; Byun et al, 2007).
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