Abstract
Previous results showed the ethyl acetate phase (SP-AcOEt), obtained from aerial parts of Solanum paludosum, relaxed the aorta isolated in the endothelium-dependent and -independent manner. The vasorelaxant effects of SP-AcOEt was not characterized on aorta rings endothelium-denuded, thus this work aimed to elucidate the mechanisms endothelium-independent vasorelaxation on rat isolated aorta. The aorta was isolated from Wistar rats and mounted in glass baths containing 6 mL of normal Krebs physiological solution with pH at 7.4. The preparation was maintained at 37ºC and bubbled continuously with a mixture of 95% O2 and 5% CO2. Aortic rings were maintained for 1 hour by a resting tension of 1g and next were contracted with phenylephrine after the sustained contraction ACh was added to access the integrity of the endothelium. SP-AcOEt relaxed pre-contracted aorta by KCl-30mM or -80mM in a similar manner, suggesting blockade CaV, but not channel-K+ participating. SP-AcOEt also inhibited the contraction induced by CaCl2 and relaxed pre-contracted aorta by (±)-BayK8644 (EC50 = 16.9±1.3 μg/mL), which confirms the involvement of L-type CaV blockade. SP-AcOEt presented vasorelaxation endothelium-independent that involves L-type CaV blockade.
Highlights
The cardiovascular diseases have been the main causes of death, hospitalization and outpatient care in the world, including developing countries, as such Brazil (GBD, 2016)
The aerial parts of the plant were collected in Brazil, State of Paraíba, municipality of João Pessoa, in summer of January 1999
The powdered aerial parts of Solanum paludosum were extracted with EtOH at room temperature
Summary
The cardiovascular diseases have been the main causes of death, hospitalization and outpatient care in the world, including developing countries, as such Brazil (GBD, 2016). The medicinal plants are used, mainly, to treat hypertension (Zago et al, 2020). The researching for newer treatments is an ongoing process and plants may be a significant source of medicines. (Solanaceae) is an herbaceous species, called “todomaka” in Surinam and “jurubeba”, “jurubeba-roxa” or “jurubeba-brava” in Brazil. The fruits from Solanum paludosum showed molluscicidal activity (Silva et al, 2005), the root bark has been reported hemolytic activity on erythrocytes of rats and displayed spasmolytic activity on rat uterus and guinea-pig trachea (Riet-Correa et al, 2011) and vasorelaxant action (Monteiro et al, 2012). The aerial parts presented antioxidant and antibacterial activity (Siqueira et al, 2011)
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