Abstract
IntroductionSufficient uterine blood supply is essential for the fetus to develop normally in the uterus. Several mechanisms are involved in the process of vessel development in deciduas and villus. We focus on whether first-trimester decidua side population (SP) cells contain cells capable of differentiating into endothelial cells.MethodsEight decidua samples were collected from healthy women, 22- to 30-years old, undergoing elective terminations of early pregnancy (six to eight gestational weeks). The cell suspensions from human deciduas were stained by Hoechst 33342 and sorted by flow cytometry, further cultured under differentiation conditions and analyzed for specific markers. These cells were implanted into ischemic limbs of nude mice to test the capacity of angiogenesis in vivo by DiI tracers and immunohistochemistry.ResultsDecidua CD31-CD146- SP cells of first-trimester human pregnancy can differentiate into endothelial cells, express the corresponding specific markers of endothelial cells, such as CD31 and CD146, and form tube-like structures on Matrigel and part of newly formed vessels in the ischemic limbs of nude mice. Vascular endothelial growth factor was more effective in promoting proliferation of CD31-CD146-SP cells compared with other growth factors, and estrogen and progesterone at a final concentration of 10 μmol/L and 30 μmol/L, respectively, promoted the migration of CD31-CD146-SP cells in a dose-dependent manner.ConclusionsCD31-CD146- SP cells may be involved in the formation of new vessels in the maternal aspect of the placenta in the first trimester.
Highlights
Sufficient uterine blood supply is essential for the fetus to develop normally in the uterus
CD31 is known to be highly expressed in endothelial progenitor cells and endothelial cells, and CD146 in smooth muscle cells and endothelial cells
vascular endothelial growth factor (VEGF), Basic fibroblast growth factor (bFGF), Epidermal growth factor (EGF), and Insulin-like growth factor (IGF) treatment enhanced proliferation of CD31-CD146-side population (SP) cells, almost two times more than in non-SP cells and the control treated with 0.2% Fetal bovine serum (FBS) only (Figures 2A-2E)
Summary
Sufficient uterine blood supply is essential for the fetus to develop normally in the uterus. Several mechanisms are involved in the process of vessel development in deciduas and villus. Sufficient nourishment is essential for a fetus to develop normally in the uterus. Evidence from several studies confirms that the vascular density of decidua tissues increases quickly in the first trimester and continues to increase slowly throughout gestation [4,5,6]. Many research studies have described the development of new blood vessels in the early placenta, especially the fetal aspects, in detail including the formation mechanism, the differentiated into endothelial cells and can be promoted by vascular endothelial growth factor (VEGF), estrogen and progesterone in vitro. SP cells in decidua may play a role in the process of new blood vessel development in the maternal placenta. We investigated the different factors required for the induction of CD31-CD146- SP cells to endothelial cells in vitro and confirmed the findings in further animal experiments
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
