Abstract
The inhibitory effect of acetone, ethanol, and aqueous extracts of ten medicinal plants on β-lactamase from Staphylococcus sciuri and Klebsiella pneumoniae was investigated in vitro by starch-iodine agar plate method. The results revealed the success of starch-iodine method for the detection of the inhibition of β-lactamase activity by the various extracts of each individual plant. The acetone extracts of Catharanthus roseus, Eucalyptus camaldulensis, and Schinus terebinthifolius induced an inhibitory effect on β-lactamase from Staphylococcus sciuri. On the other hand, acetone extracts from only Eucalyptus camaldulensis, and Schinus terebinthifolius expressed strong inhibitory effect on β-lactamase from Klebsiella pneumoniae. The acetone extracts expressed the highest inhibition for β-lactamases activity compared to ethanolic and aqueous extracts which exhibited appreciable inhibitory effect. β-lactamase from S. sciuri was inhibited by extracts from C. roseus, E. camaldulensis and S. terebinthifolius whereas β-lactamase from K. pneumoniae was inhibited only by extracts from E. camaldulensis and S. terebinthifolius.
Highlights
The inhibitory effect of acetone, ethanol, and aqueous extracts of ten medicinal plants on βlactamase from Staphylococcus sciuri and Klebsiella pneumoniae was investigated in vitro by starch-iodine agar plate method
The results showed that extracts from three plants (C. roseus, E. camaldulensis and S. terebinthifolius) expressed an inhibitory effect on βlactamase originated from S. sciuri
In the present investigation, starch-iodine agar plate was a screening method adopted for the detection of β-lactamase inhibitors
Summary
The inhibitory effect of acetone, ethanol, and aqueous extracts of ten medicinal plants on βlactamase from Staphylococcus sciuri and Klebsiella pneumoniae was investigated in vitro by starch-iodine agar plate method. Other antimicrobial bacterial agents are necessary to be advanced to control bacteria with multi-drug resistant To face this challenge, there has been increasing interests to discover antimicrobial agents from medicinal plant extracts as an alternate strategy [4, 5, 6]. The application of β-lactamase inhibitors coupled with β-lactam antibiotic is the foremost valuable strategy to treat a range of infections. The role of these enzyme inhibitors is the inhibition of the β-lactamase in the periplasmic space. Several publications described activity of medicinal plants as β-lactamase inhibitors [9] either as extracts or products [10, 7, 2]
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