Abstract
Summary Pea (Pisum sativum, cv. Weibulls Marma) stem cuttings were rooted in nutrient solution. The development of root primordia at the base of the cuttings was followed microscopically. The levels of zeatin-type and isopentenyladenosine-type cytokinins were determined in bleeding sap and in extracts of the lowermost centimeter of the stem base with an enzyme-linked immunosorbent assay (ELISA). Extracts were purified with the help of immunoaffinity chromatography. The bleeding sap as well as the tissue contained cytokinins of both types. In the stem tissue the level of the cytokinins decreased during the day after the cuttings were made. They remained at low levels for 3-4 days during the early phases of primordial development and increased later when the primordia began elongating. The results support the hypothesis that endogenous cytokinins are involved in the regulation of adventitious root formation.
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