Abstract
In vitro cloned lines derived from a primary nickel-induced rat rhabdomyosarcoma exhibited diverse levels of susceptibility to spontaneous NK activity. The presence of NK target structures was revealed by competition assays on all cloned cell lines, and the NK susceptibility of the tumour lines varied according to their osmotic fragility. Tumour cell lines derived from metastatic lung nodules presented similar NK susceptibilities to cells originating from the primary tumour. However, cloned cell lines differed in their capacity to form lung colonies after i.v. injection, and in their potential for invading lungs after s.c. primary tumour development. No correlation was found between lung colonization potential and NK resistance. Studies of the correlation between metastatic potential and NK sensitivity revealed that (1) all the NK resistant tumour cells were highly metastatic; (2) NK susceptible tumour cells could be either highly or weakly metastatic. Therefore, highly metastatic tumour cells could be either resistant or susceptible to NK lysis. We conclude that the property of resistance to NK contributes to a high metastatic potential. However, other properties could counterbalance and finally prevail over NK susceptibility thus enabling NK susceptible cell lines to be also highly metastatic.
Highlights
Splenic lymphocytes from the 3 month-old female WAG rats were used a source of NK effector cells, the lysis of YAC cells being used as a control of lytic activity
The two cell lines derived from metastatic lung nodules were susceptible to NK lysis
In this study we have demonstrated that the NK susceptibilities of different cell lines derived from a single primary tumour are heterogeneous
Summary
Ten to 14 week-old female WAG rats were used as NK donors, unless otherwise specified. Similarlyaged male WAG rats were used as recipients of tumour cells, all rats being bred at the Institut de Recherches Scientifiques sur le Cancer (Villejuif, France) under SPF conditions. The primary rhabdomyosarcoma appeared at the site of i.m. injection of 10mg of Nickel (Prolabo, France) in the thigh of a WAG male rat. When the tumour reached 30mm in diameter, it was minced in PBS and dissociated in a 0.25% trypsin in PBS solution. The parental tumour cells, denoted as 94/0, were cultivated in Dulbecco's modified Eagle's.
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