Value of sTREM-1, procalcitonin and CRP as laboratory parameters for postmortem diagnosis of sepsis

Abstract

Triggering receptor expressed on myeloid cells-1 (TREM-1) was reported to be up-regulated in various inflammatory diseases as well as in bacterial sepsis. Increased cell-surface TREM-1 expression was also shown to result in marked plasma elevation of the soluble form of this molecule (sTREM-1) in patients with bacterial infections. In this study, we investigated sTREM-1, procalcitonin and C-reactive protein in postmortem serum in a series of sepsis-related fatalities and control individuals who underwent medico-legal investigations. sTREM-1 was also measured in pericardial fluid and urine. Two study groups were prospectively formed, a sepsis-related fatalities group and a control group. The sepsis-related fatalities group consisted of sixteen forensic autopsy cases. Eight of these had a documented clinical diagnosis of sepsis invivo. The control group consisted of sixteen forensic autopsy cases with various causes of death. Postmortem serum sTREM-1 concentrations were higher in the sepsis group with a mean value of 173.6pg/ml in septic cases and 79.2pg/ml in control individuals. The cutoff value of 90pg/ml provided the best sensitivity and specificity. Pericardial fluid sTREM-1 values were higher in the septic group, with a mean value of 296.7pg/ml in septic cases and 100.9pg/ml in control individuals. The cutoff value of 135pg/ml provided the best sensitivity and specificity. Mean urine sTREM-1 concentration was 102.9pg/ml in septic cases and 89.3pg/ml in control individuals. Postmortem serum sTREM-1, individually considered, did not provide better sensitivity and specificity than procalcitonin in detecting sepsis. However, simultaneous assessment of procalcitonin and sTREM-1 in postmortem serum can be of help in clarifying contradictory postmortem findings. sTREM-1 determination in pericardial fluid can be an alternative to postmortem serum in those situations in which biochemical analyses are required and blood collected during autopsy proves insufficient.