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Abstract
Chloropropanols and related substances have received a great deal of attention in the world. Regulation values have already been established in the EU for infant formula. There are several validated analytical methods for these substances, such as the AOAC official method. However, no analytical official method has been established for infant formula in Japan. In this study, the AOAC official method was improved to a more versatile splitless injection method in order to make it feasible for many laboratories. The results of the validation data for the improved method with optimized injection conditions were as follows. The limit of quantification was 10 μg/kg, the recovery rate ranged from 81.6 to 114.5%, and the intermediate precision ranged from 1.6 to 7.6%. These results were adapt to AOAC and EU requirement and demonstrated the good validity of the improved method.
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