Abstract
A fast method for the identification and stability evaluation of the aggregation pheromone rhynchophorol, which is the main substance used for chemical communication by the beetle Rhynchophorus palmarum L., was validated. In addition, the technique was applied to the evaluation of two inorganic matrices, with the objective of using them as controlled-release devices. The analytical method showed good linearity (R2 = 0.9978), precision (CV% < 1.79), recovery (84–105%) and limits of detection (0.2 mg mL−1) and quantification (0.3 mg mL−1); in compliance with the validation legislation established by ANVISA. In the interaction study, the inorganic matrices zeolite L and Na-magadiite showed high rates of pheromone recovery without promoting its degradation for a period of 180 days, which is not reported in the literature for other matrices. The structures of the zeolite L/rhynchophorol and Na-magadiite/rhynchophorol composites showed slower release kinetics during the storage period when compared with pure pheromone, which is desirable since it extends the period of rhynchophorol release and decreases the negative effects caused by the environmental parameters.
Highlights
IntroductionThe beetle Rhynchophorus palmarum L. is an insect of the family Dryophthoridae, subfamily Rhynchophorina and class Rhynchophorini [1]
The beetle Rhynchophorus palmarum L. is an insect of the family Dryophthoridae, subfamily Rhynchophorina and class Rhynchophorini [1].This insect is a recurrent pest, which attacks mainly sugarcane (Saccharum officinarum) and coconut (Cocos nucifera L.) plantations, damaging the stalks of these plants in the search for food and reproduction sites, and laying eggs which will later hatch [2]
The results show CV and standard deviation (STD) values lower than 5%, satisfying the requirements established by ANVISA [16]
Summary
The beetle Rhynchophorus palmarum L. is an insect of the family Dryophthoridae, subfamily Rhynchophorina and class Rhynchophorini [1]. This insect is a recurrent pest, which attacks mainly sugarcane (Saccharum officinarum) and coconut (Cocos nucifera L.) plantations, damaging the stalks of these plants in the search for food and reproduction sites, and laying eggs which will later hatch [2]. The highest risk posed by this beetle is its use as a vector by the nematode Bursaphelenchus cocophilus. This nematode is the main agent responsible for causing the disease in. The use of these natural enemies is not effective against all insects of
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