Abstract
BackgroundSimple, sensitive, free of organic solvents, kinetic spectrophotometric method has been developed for the determination of Clindamycin Hydrochloride, both in pure form and Capsules. Method is based on reaction of Clindamycin with potassium iodide and potassium iodate in an aqueous medium at (25 ± 2 °C) to produce yellow-coloured tri iodide ions (I3−). The reaction is followed spectrophotometrically by measuring the absorbance at wavelength 350 nm during 40 min.ResultsThe effects of analytical parameters on reported kinetic methods were investigated. Under the optimized conditions, the initial rate and fixed time (at 10 min) methods were used for constructing the calibration graphs. The graphs were linear in concentration ranges 1–20 μg ml−1 with limit of detection of 0.12 and 0.22 μg ml−1for the initial rate and fixed time methods, respectively. The results were satisfactory and the analytical performance for both methods was validated.ConclusionThe proposed methods have been applied to determine the components in capsules with an average recovery of 98.25–102.00% and the results are in good agreement with those found by the reference method.
Highlights
IntroductionBy binding to the 50S subunit of the ribosome, Clindamycin inhibits the synthesis of bacterial proteins
Clindamycin (CLN),1-methyl-4-propyl-L-prolyl]amino}-1-thio-Lthreo-3-Dgalacto-octopyranoside), is a semi-synthetic analog of lincomycin [1]. (Fig. 1).By binding to the 50S subunit of the ribosome, Clindamycin inhibits the synthesis of bacterial proteins
There are a variety of analytical techniques to determine Clindamycin in various pharmaceutical formulations, as well as in biological samples
Summary
By binding to the 50S subunit of the ribosome, Clindamycin inhibits the synthesis of bacterial proteins. It is active against Gram-positive aerobic, anaerobic and some Gram-negative aerobic bacteria. There are a variety of analytical techniques to determine Clindamycin in various pharmaceutical formulations, as well as in biological samples. High-performance liquid chromatography (HPLC) is commonly used [4,5,6,7,8,9], potentiometric determination [10] capillary electrophoresis [11], micellar chromatography [12], chemiluminescence methods [13], voltammetry [14,15,16] and spectrophotometric determination that dependent on color forming [17, 18]. Sensitive, free of organic solvents, kinetic spectrophotometric method has been developed for the determination of Clindamycin Hydrochloride, both in pure form and Capsules. The reaction is followed spectrophotometrically by measuring the absorbance at wavelength 350 nm during 40 min
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