UvHrip1, an effector secreted by Ustilaginoidea virens, suppresses basal defense and promotes disease development in Arabidopsis thaliana.

Abstract

Rice false smut (RFS), caused by Ustilaginoidea virens, is one of the most detrimental rice fungal diseases and pose a severe threat to rice production and quality. Effectors in U. virens often act as a set of essential virulence factors that play crucial roles in the interaction between host and the pathogen. Thus, the functions of each effector in U. virens need to be further explored. Here, a conserved small secreted hypersensitive response-inducing protein (hrip) was named UvHrip1. Functional validation was investigated to prove that UvHrip1 suppressed cell death symptom and ROS accumulation in Nicotiana benthamiana triggered by Burkholderia glumae. We performed transgenic technology to demonstrate UvHrip1 remarkably inhibited pathogen-associated molecular pattern-induced defense responses in Arabidopsis seedlings and plants, including the expression of defense-response genes. Furthermore, disease progression caused by the type III secretion system-defective mutant from Pseudomonas syringae pv. tomato DC3000 was strongly facilitated in transgenic Arabidopsis ectopic expressing UvHrip1. Our data demonstrated UvHrip1 suppresses plant innate immunity and promoting disease multiplication in Arabidopsis.