Abstract

Abstract The relationship between the amount of phosphorous supplied and nitrogen fixation by free living nitrogen fixing organisms like Azotobacter and Clostridium has been established among others by Green (2). A quantitative relationship between the availability of phosphates and nitrogen fixation by Azotobacter has been expressed by several workers (Stoklassa (6) ; Waksman and Karunakar (7) ; Singh and Shrikhande (4); Sen et. al. (5)). In the case of a symbiotic organism like Rhizobium, phosphorous has been considered essential for the normai nodule growth and increased race of infection by Anderson and Thomas (1). The intimate relationship observed between phosphate supply and nitrogen fixation in the case of free living organism might cause one to suspect that the more efficient strains of Rhizobium spp. might have also higher capacities of phosphate utilization.

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