Abstract
The current study illustrates the synergetic effect of both environment-friendly green synthesized zinc oxide nanoparticles (ZnO-NPs) of Ocimum basilicum extract using the bio-reduction process and bacteriophages (phage) to control pathogenic bacteria. Ocimum basilicum has various phytochemical compounds that were responsible for the formation of ZnO-NPs. In addition, the phage ZCSE6, as a biological material, has a bacteriolytic activity against pathogenic bacteria. The spectrum of UV–Vis indicated a corresponding ZnO-NPs peak at 370 nm. Transmission electron micrographs (TEM) showed irregular particles in shape with 10–25 nm in size. Furthermore, the ZnO-NPs displayed biofilm deformation that can be formed by Staphylococcussciuri(S. sciuri). Moreover, it has a synergic antibacterial activity against Salmonella enterica (S. enterica) when combined with phage ZCSE6. This study reveals the antibacterial effect of using metallic and biological materials and their possible applications in the medical field.
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