Abstract

Recently, significant progress has been made in the technological development of bioreactor and bio-fuel cell. In rural and remote areas, bio-fuel cell can be a practical technique for the conversion of waste and wastewater into biomass energy while bio-reactors can treat domestic and organic waste and wastewater. The bio-reactor must be seeded with photo-synthetic or hydrogenation bacteria, and must be coupled with a bio-fuel cell which is one of the hottest research areas in the world, presently. Finally, the bio-reactor and bio-fuel cell processes must be controlled by a monitoring system. The objective of the present experiment was to test the amperage and voltage production capability of a bio-reactor coupled with a bio-fuel cell (PEFC), operated under variable conditions. The mesophilic clostridium sp. (BX2) was isolated from cow manure and then used as inoculum to seed the bio-reactor for hydrogen production which was measured by Micro GC. The hydrogen concentration exceeded over 70 %, when steady state was reached. The bio-fuel cell, operated under a lower pressure of 3kPa above atmosphere and generated a steady state current and voltage of 3.5mA and 0.65 volts. The mean power generation was 24.57W. The finite element analysis method was employed to calculate the hydrogen diffusion in the anode. The system was able to function using highly concentrated organic wastewater.

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