Abstract
This experiment aimed to compare at day seven after ovulation, the protein profile of uterine fluid in cyclic mares with mares infused two days before with Day 13 conceptus fragments. Experimental animals were ten healthy cyclic mares, examined daily to detect ovulation (Day 0) as soon as estrus was confirmed. On day seven, after ovulation, uterine fluid was collected, constituting the Cyclic group (n = 10). The same mares were examined in the second cycle until ovulation was detected. On day five, after ovulation, fragments from a previously collected concepti were infused into each mare's uterus. Two days after infusion, uterine fluid was collected, constituting the Fragment group (n = 10). Two-dimensional electrophoresis technique processed uterine fluid samples. A total of 373 spots were detected. MALDI-TOF/TOF and NanoUHPLC-QTOF mass spectrometry identified twenty spots with differences in abundance between the Cyclic and Fragment group. Thirteen proteins were identified, with different abundance between groups. Identified proteins may be related to embryo-maternal communication, which involves adhesion, nutrition, endothelial cell proliferation, transport, and immunological tolerance. In conclusion, conceptus fragments signalized changes in the protein profile of uterine fluid seven days after ovulation in comparison to the observed at Day 7 in the same cyclic mares.
Highlights
The establishment and maintenance of pregnancy are critically dependent on embryomaternal communication during the preimplantation period (Klein and Troedsson, 2011a)
It is hypothesized that the infusion of conceptus fragments promotes changes in the content of uterine fluid protein. This experiment aimed to compare at day seven after ovulation, the protein profile of uterine fluid in cyclic mares with mares infused two days before with 13th day conceptus fragments
The protein concentration recovered from uterus showed a median of 2.36 ± 0.21 μg/μL for the Cyclic group and 2.5 ± 0.3 μg/μL for the Fragment group, without differences between groups (P = 0.255)
Summary
The establishment and maintenance of pregnancy are critically dependent on embryomaternal communication during the preimplantation period (Klein and Troedsson, 2011a). Thereupon, the conceptus-derived pregnancy recognition signal has not been identified (Klein and Troedsson, 2011b). Histomorphometric (Keenan et al, 1987; Caballeros et al, 2019; Camozzato et al, 2019) and Copyright © The Author(s).
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