Abstract
A study was carried out to see whether the near-black down of an eumelanotic columbian phenotype (eWh/eWh, Co/Co, Ml/Ml) could be used in color sexing crosses as a background for the expression of the sex-linked barring (B) gene. Barred (B) and nonbarred (b+) alleles were accurately identified on a heavily eumelanotic columbian phenotype, which provides a brown-faced black down for the B-induced white head spot. Color sexing was possible due to the association between barred males (B/b+) and white head spot. Average accuracy obtained was 97%. Sexing was not possible in the presence of heterozygous Ml/ml+.
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