Abstract
Simple SummaryAccording to the available literature, high prolificacy in sheep breeds could be caused by differences in many genes (polygenic trait) or as in some sheep breeds to a difference in one gene such as BMP-15. This paper describes the use of the High-Resolution Melting method in quick identification of the known mutation in the BMP-15 gene, which affects high prolificacy in the Olkuska breed of sheep.Olkuska is a highly prolific sheep breed in Poland. Thanks to earlier identification of the genetic basis of its prolificacy, a mutation in the BMP-15 gene, we can use molecular biology tools to identify this causative mutation affecting prolificacy. In our research, we used the High-Resolution Melting (HRM) and Sanger sequencing methods to identify the genotypes of the studied animals. The result obtained by the HRM method is identical to those obtained by the sequencing method, which confirms the effectiveness of the HRM method and the possibility of quick and cheap identification of individuals with a FecXO mutation.
Highlights
Sheep play an important role in modern agriculture
In the mutation present in Olkuska sheep, which can increase the attractiveness of molecular tools in sheep breeding study, we propose a low cost, quick and sensitive High-Resolution methodprogram for and allow for more breeding
To validate the results obtained from High-Resolution Melting (HRM) analysis all 100 samples were sequenced (Figure 3)
Summary
Sheep play an important role in modern agriculture. Reproductive traits are especially crucial for sheep production because they determine the profitability of breeding [1,2,3]. They are characterized by low heritability, and selection based on phenotypic values is lengthy and inefficient. Genotype-based animal selection appears to be much more effective to improve prolificacy in sheep [1,4,5]. A number of prolificacy genes such as BMPR-1B (bone morphogenetic protein receptor 1B), BMP-15 (bone morphogenetic protein 15), GDF-9 (growth differentiation factor 9) and B4GALNT2
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