Abstract
Temperature-sensitive gels (cross-linked, partially hydrolyzed polyacrylamide gels) with the property to absorb solutes less than 1nm in diameter at 4°C and collapse at higher temperatures (190°C) were used to concentrate Escherichia coli from 2% low-fat milk. Milk samples seeded with bacteria were reduced eight-fold in volume with average recoveries of 40–66% at an optimal pH of 5.5. Repeated use of the gel did not affect its efficiency.
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