Abstract
Reverse-phase chromatography has been used for carrying out Maxam-Gilbert sequencing reactions. The DNA loss has been minimized. Time-consuming ethanol precipitation and lyophilization of piperidine have been eliminated. The method has been used successfully in sequencing oligonucleotides as well as large DNA fragments. Based upon these modifications, we have developed a semiautomatic device for carrying out the Maxam-Gilber sequencing reactions. It is also possible to fully automate the Maxam-Gilbert method of DNA sequencing, and we present the diagram of a fully automated set up. The development of such a machine should expedite the sequencing of the large number of genes being cloned.
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