Abstract
The objective of this work was the vegetative rescue of Cedrela odorata L. adult trees from forest areas by micropropagation using shoot regeneration from axillary buds in vitro. Nodal segments (0.5 and 1.0 cm) of shoots formed from cuttings taken from canopy sprouts were used. The explants were successfully established in vitro on MS medium supplemented with 2.22 ?M BAP, 0.49 ?M AIB, 0.28 ?M GA3 and PPM (0 and 8.6 ?M). Shoot multiplication on MS with combinations of GA3 (0.28 and 1.12 ?M) and BAP (2.2, 3.3, 4.4, 6.6, and 8.8 ?M) and shoot rooting on medium with 4.9 ?M AIB, 5.7 ?M AIA or 5.37 ?M ANA were evaluated. The MS medium supplemented with 0.28 ?M GA3 and 8.8 ?M BAP generated 4.16 shoots per explant. The best rooting induction was observed on medium containing 4.9 ?M IBA, resulting in 60% of shoot rooting. The plantlets rooted were acclimatized and showed normal development with 97% survival rate. The use of canopy sprouts as explants is feasible in the rescue of C. odorata and the combination of BAP and GA3 favors in vitro multiplication.
Highlights
Induction and regeneration of shoots in vitro Regeneration of shoots under in vitro conditions was possible using material rescued from C. odorata matrices
Silveira et al (2016) showed that using 0.4 and 0.8% PPM in WPM culture medium eliminated bacterial contamination and the 0.8% dose reduced fungal contamination to 2% in nodal segments obtained from 1-2 year old seedlings of Calophyllum brasiliense
Robert et al (2020), in vegetative rescue of C. odorata using nodal segments obtained from grafted material observed higher regeneration compared to the use of cuttings buds under in vitro conditions
Summary
Due to the economic importance of the wood of C. odorata, the extractive exploitation, which added to the low regeneration capacity, has taken the species to the category of endangered (Brasil, 2014). Another limiting factor to the establishment of commercial C. odorata forests is the presence of the insect Hypsipyla grandella (Zeller, 1848) (Lepidoptera: Pyralidae) which damages the tree’s growing points, compromising the upright development of the stem as well as the development and growth of young trees (Castro et al, 2018). According to Alvim et al (2020), in forest species with low propagative rate by seeds, micropropagation enables in vitro conservation of invigorated material, mass multiplication and the selection of genetically superior matrices
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