Use of microcalorimetry for the characterization of marine metabolic activity at the water-sediment interface

Abstract

Metabolic processes occurring at the sea-water-sediment interface were studied using a circulation flow microcalorimeter. A methodology was developed to characterize rapid and global changes in metabolism and energy flow, not easily detectable with reductionist approaches. Sea water was pumped continuously, 5–10 mm above the sediment, in experimental microcosms; a 100-μm filter prevented passage of meiofauna. This “circulating interface” was taken through the microcalorimeter and from there to an oxygen electrode, and was returned to the microcosm. The microcosms were experimentally eutrophicated using peptone (4 mg·ml −1). The relationship between heat production and oxygen tension in the circulating interface has been compared with ATP production, 14CO 2 and [ 14C]particulate matter turnovers. Initial heat steady-state production rises to a peak of 130 to 180 μW·ml −1 in 6 to 8 h after peptone treatment. The microcalorimetric peak is closely correlated with 14CO 2 turnover and partially correlated with micro-events on the pO 2 curve. ATP concentration and particulate- 14C turnover increase constantly and then stabilize, with the establishment of a new heat production steady state. The approach provides an indication of the temporal behaviour of complex mixtures of microorganisms and ciliates at the water-sediment interface, and gives holistic measurements of energy flow after induced perturbation (eutrophication) of the ecosystem. Although many problems remain to be solved in this field, it is shown here that flow microcalorimetric measurements can be used to monitor the effects of addition of reagents like pollutants and nutrients.