Abstract
In this work, novel dendrons (1J and 2J) were prepared as ferrocene core by means of divergent method. And the expanded second generation dendrons including Pd(II) and Pt(II) ions was synthesized from 2J. Synthesized all compounds were characterized by molar conductivity, magnetic susceptibility, thermal analyses (TGA), FT-IR, UV–Vis, 1H NMR, AAS, LC-MS methods. Then, glucose oxidase (GOx) enzyme was immobilized on them (2J and expanded 2J). Their optimization parameters were determined separately for free enzyme, 2J-immobilized and expanded 2J-immobilized. Immobilized 2J’s palladium complex displayed excellent reusability in cycle analysis. Furthermore, determination of glucose in artificial urine was investigated as catalytic. The results showed that presence of glucose in artificial urine may be determined using to immobilized-dendrons. This research is the first example of immobilization of GOx over dendrons with Ferrocene-Stem.
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