Urbanization Imprint on Soil Bacterial Communities in Forests and Grasslands

Abstract

Urbanization alters land uses and creates heterogeneous environmental conditions in cities and their surroundings, which may directly or indirectly impact soil microorganisms. However, how urbanization affects soil bacterial diversity and community composition, particularly in different land use types, remains largely unknown. In this study, we collected 36 soil samples (18 forest and 18 grass soils) along a rural-suburban-urban gradient in Chang-Zhu-Tan agglomeration. The bacterial diversity and community composition were investigated using 16S rRNA gene sequencing that targeted the V3-V4 region. Our results showed that urbanization induced shifts in bacterial diversity and community composition in both forestlands and grasslands. Specifically, soil bacterial diversity was higher in urban areas than in their suburban and rural counterparts in forests and grasslands, particularly in forests, where significant increases were detected. Urbanization changed the most dominated soil bacterial community from Acidobacteria to Proteobacteria in forestland. Significant decrease and increase were observed in the relative abundance of Acidobacteria (e.g., Acidobacteriales, Acidobacteriia_Subgroup2 and Solibacterales) and Proteobacteria (e.g., Betaproteobacteriales, Myxococcales and Sphingomonadales), respectively, in the forests with increasing urbanization intensity. In contrast, Proteobacteria always dominated the soil bacterial community along the rural-suburban-urban gradient in grassland, and significant decrease and increase in Nitrospirae and Latescibacteria were induced by urbanization, respectively. In addition to urbanization and total nitrogen, total organic carbon and ratio of carbon and nitrogen were the main factors that related with the bacterial community in forest soils, whereas soil water content was the main factor related with soil bacterial community in the grasslands. Together, our results indicate that the urbanization results in shifts in bacterial community composition and diversity, but the extent varied between forest and grassland, which may due to different human management intensity.