Uptake, Translocation, and Subcellular Distribution of Azoxystrobin in Wheat Plant ( Triticum aestivum L.).

Abstract

The uptake mechanism, translocation, and subcellular distribution of azoxystrobin (5 mg kg-1) in wheat plants was investigated under laboratory conditions. The wheat-water system reached equilibrium after 96 h. Azoxystrobin concentrations in roots were much higher than those in stems and leaves under different exposure times. Azoxystrobin uptake by roots was highly linear at different exposure concentrations, while the bioconcentration factors and translocation factors were independent of the exposed concentration at the equilibrium state. Dead roots adsorbed a larger amount of azoxystrobin than fresh roots, which was measured at different concentrations. Azoxystrobin preferentially accumulated in organelles, and the highest distribution proportion was detected in the soluble cell fractions. This study elucidated that the passive transport and apoplastic pathway dominated the uptake of azoxystrobin by wheat roots. Azoxystrobin primarily accumulated in roots and could be acropetally translocated, but its translocation capacity from roots to stems was limited. Additionally, the uptake and distribution of azoxystrobin by wheat plants could be predicted well by a partition-limited model.