Abstract
The rate of 2-deoxyglucose uptake is approximately three times faster in actively growing BALB 3T3 cells than in resting cells, and another three times faster in polyoma or SV40-transformed cells than in actively growing untransformed cells. Uptake of 2-deoxyglucose increases approximately 10-fold after infection of resting cells by polyoma virus, and 3-fold after infection of growing cells. Uptake of 3- O-methylglucose also increases after infection of resting cells, showing that changes in the rate of 2-deoxyglucose uptake can be ascribed to changes in the rate of transport of hexose across the cell membrane. The increased rate of uptake in polyoma-infected cells begins about 12 hr after infection of resting cells at 37°, and occurs in the presence of cytosine arabinoside. The increase in uptake occurs after infection at 39° by a polyoma temperature-sensitive mutant, ts25, which is blocked in viral DNA synthesis at the restrictive temperature. The increase does not occur after infection with ts3, a mutant which is blocked in induction of cellular DNA synthesis, showing that an early viral gene product is required for increased uptake.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.