Abstract
Black sea bream (Mylio macrocephalus) were adapted to salinities of 0 ppt (freshwater), 6 ppt (hypo-osmotic), 12 ppt (iso-osmotic), 33 ppt (seawater), and 50 ppt (hypersaline) for 1 month. Using RT-PCR assays, the expression of pituitary growth hormone (GH) and hepatic insulin-like growth factor 1 (IGF-1) genes were studied. It was found that the transcripts for both of these genes were highest in fish maintained at iso-osmotic salinity. To correlate the expression of GH and IGF-1 with an index of growth, we also measured the transcript levels of glucose-6-phosphate dehydrogenase (G6PDH) in liver. It was found that this transcript was also elevated in iso-osmotically adapted black sea bream.
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