Abstract
BackgroundOvarian cancer (OC) is associated with high mortality in gynecological oncology; this is mainly due to the low diagnosis rate. Exosomal miRNA has demonstrated potential as a tumor biomarker. We aimed to explore the diagnostic potential of serum exosomal miR-1307 and miR-375 for OC.MethodsThe first six candidate miRNAs were selected from the previous literature. The relative quantification of qRT-PCR was used to screen for the stability of exosomal miRNAs, followed by validation of the cohort. ROC analysis was employed to analyze the specificity and sensitivity of exosomal miRNA.ResultsMiR-1307 and miR-375 were confirmed stably existing in serum exosomes of OC. Moreover, miR-1307 and miR-375 were both significantly up-regulated in serum exosomes of OC compared to ovarian benign and healthy groups. The overexpressed miRNAs showed independent diagnostic power and enhanced the diagnostic accuracy of traditional biomarkers when combined with CA-125 and HE4. MiR-1307 was associated with tumor staging, and miR-375 was associated with lymph node metastasis of OC.ConclusionOur results suggest that serum exosomal miR-1307 and miR-375 could serve as potential tumor biomarkers to improve diagnostic efficiency for OC.
Highlights
Ovarian cancer is a serious gynecologic disease
Transmission electron microscopy (TEM) analysis revealed the round shape of exosomes that were derived from serum (Fig. 1d) and that the particle diameter observed in the photographs was in accord with the Nanoparticle-tracking analysis (NTA)
Combined with the results of the various methods confirmed the successful extraction of serum exosomes
Summary
Ovarian cancer is a serious gynecologic disease. The incidence of OC is the second highest among gynecological tumors, accounting for 3% of all tumors. The mortality rate is approximately 5%, meaning that about 225,500 new cases are reported annually, resulting in over 140,200 deaths worldwide [1,2,3]. The conventional serum tumor biomarker CA125 combined with abdominal and pelvic, transvaginal ultrasonography were (2019) 12:6 prognosis of more than 90% five-year survival [12]. New diagnostic strategies continue needed to be explored to meet the challenge of OC. Ovarian cancer (OC) is associated with high mortality in gynecological oncology; this is mainly due to the low diagnosis rate. Exosomal miRNA has demonstrated potential as a tumor biomarker. We aimed to explore the diagnostic potential of serum exosomal miR-1307 and miR-375 for OC
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
