Abstract
Objective To establish an UPLC finqerprints of Potentilla discolor from different habitats and provide a scientific basis for its quality control. Methods The chromatographic condition was as follow: ACQUITY BEH C18 Column ( 2.1 mm × 100 mm, 1.7 μm) eluted with the mobile phases of methanol, acetonitrile, and 0.2% phosphoric acid water in gradient mode. The flow rate was 0.40 ml•min-1 and the detection wavelength was set at 254 nm. Results The mutual mode of fingerprints of 20 batches Potentilla discolor was established. There were 15 common peak; and the similar degrees of the 16 samples to the common mode were between 0.628 and 0.996. The 16 batches of Potentilla discolor can be gathered into 4 categories through cluster analysis. The PCA value of each fingerprint peak was calculated; 4 principal components accounted for over 88.874%; the total variance were extracted from the original data. Conclusion This method is quick and easy and could be used to evaluate the quality of Potentilla discolor. Key words: Potentilla discolor; different habitats; UPLC; chromatographic fingerprint; cluster analysis; PCA
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
