Abstract
In this article, we have explored the influence of a peculiar member of imidazolium-based ionic liquid (IL), 1-allyl-3-methylimidazolium chloride ([Amim][Cl]), on the stability of hemoglobin (Hb) by using fluorescence, thermal fluorescence, ANS fluorescence, circular dichroism (CD) spectroscopy, and dynamic light scattering (DLS) measurements. In the attempt of searching ILs which can provide stability to Hb, for the first time, we have successfully shown the stability of Hb in [Amim][Cl]. Our results show that [Amim][Cl] stabilizes the Hb native structure in a concentration dependent manner, which indicates that the concentration of IL is very crucial for determining the stabilization/destabilization of the protein in ILs. Here, we have observed that a low concentration of IL provides stability to the protein, while a high concentration destabilizes the protein. The commendatory results obtained from the multi-spectroscopic approaches provided some guidance regarding the mechanism of interaction between ...
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