Understanding of the Off-On Response Mechanism in Caged Fluorophores Based on Quantum and Statistical Mechanics.

Abstract

For many years, numerous fluorescent probes have been synthesized and applied to visualize molecules and cells. The development of such probes has accelerated biological and medical investigations. As our interests have been focused on more complicated systems in recent years, the search for probes with sensitive environment off-on response becomes increasingly important. For the design of such sophisticated probes, theoretical analyses of the electronically excited state are inevitable. Especially, understanding of the nonradiative decay process is highly desirable, although this is a challenging task. In this study, we propose an approach to treat the solvent fluctuation based on the reference interaction site model. It was applied to selected bioimaging probes to understand the importance of solvent fluctuation for their off-on response. We revealed that the this switching process involves the nonradiative decay through the charge transfer state, where the solvent relaxation supported the transition between excited and charge transfer states. In addition, energetically favorable solvent relaxation paths were found due to the consideration of multiple solvent configurations. Our approach makes it possible to understand the nonradiative decay facilitated by a detailed analysis and enables the design of novel fluorescent switching probes considering the effect of solvent fluctuation.