Abstract
InDictyostelium discoideumAx-2 cells, a specific point (PS-point) in the cell cycle from which they initiate differentiation in response to starvation has been specified. Using synchronized Ax-2 cells and the differential display method, a novel gene (differentiation-associated gene2;dia2) was isolated as one of the genes expressed specifically during the shift of Ax-2 cells from growth to differentiation. Thedia2gene codes a lysine- and leucine-rich protein with a predicted molecular mass of 16.9 kDa. Northern blot analysis has shown that thedia2mRNA, of 0.7 kb, accumulates in differentiating cells starved just before the PS-point, while there is no detectable expression in vegetatively growing cells. Antisense-mediated gene inactivation ofdia2greatly inhibited the progress of differentiation, presumably through the reduced expression of cAMP receptor 1 (car1). Thus, theDIA2expression was suggested to have an essential role in the initiation of differentiation, closely relating to the cAMP signaling system.
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