Abstract
With the increase in life expectancy, reducing the visible signs of skin aging has become a major issue. A reduction in collagen and hyaluronic acid synthesis by fibroblasts is a feature of skin aging. The green seaweed, Ulva intestinalis, is an abundant and rich source of nutrients, especially proteins and peptides. The aim of this study was to assess the potential cosmetic properties of a protein fraction from Ulva intestinalis (PROT-1) containing 51% of proteins and 22% of polysaccharides, and its enzymatic peptide hydrolysates on human dermal fibroblasts. PROT-1 was extracted using a patented acid- and solvent-free process (FR2998894 (B1)). The biochemical characterization and chromatographic analysis showed a main set of proteins (25 kDa). To demonstrate the anti-aging potential of PROT-1, fibroblast proliferation and collagen and hyaluronic acid production were assessed on fibroblast cell lines from donors aged 20 years (CCD-1059Sk) and 46 years (CCD-1090Sk). PROT-1 induced a significant increase in collagen and hyaluronic acid production per cell, and a reduction in cell proliferation without increasing cell mortality. These effects were reversed after protein hydrolysis of PROT-1, showing the central role of proteins in this promising anti-aging property.
Highlights
IntroductionPeople have started to pay more attention to skin health and beauty
In recent years, people have started to pay more attention to skin health and beauty
We developed a hydrolysis procedure of the obtained protein fraction (PROT-1) to produce a protein fraction of reduced molecular weight (MW), H-PROT-1, in order to assess the influence of MW on collagen and hyaluronic acid production by normal human dermal fibroblast (NHDF) cell lines from donors aged 20 and 46 years
Summary
People have started to pay more attention to skin health and beauty. Skin aging is a complex biological process that can be divided into two basic processes, intrinsic and extrinsic aging. Intrinsic aging is genetically inherited (genetic, hormonal and metabolic processes, cellular metabolism); extrinsic aging is due to external factors such as air pollution, toxins, nicotine consumption, lifestyle influence, chemicals and chronic light exposures, which contribute to accelerate its consequences [2]. One of the major consequences of skin aging is a reduction and alteration of extracellular matrix (ECM) components such as elastin, collagen and hyaluronic acid (HA) [3]. For this reason, maintaining collagen and HA levels in the dermis is essential to maintain a healthy skin
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