Abstract
目的 探索超微超顺磁性氧化铁粒子(USPIO)增强MRI监测兔抗原诱导性膝关节炎的可行性及理想成像序列.方法 9只雌性新西兰大白兔,后腿右膝关节(简称右膝关节)腔注入甲基化小牛血清蛋白(mBSA)0.5 ml(2 mg/ml)诱导关节炎模型,后腿左膝关节(简称左膝关节)作为对照.另选3只制成假模型组.模型成功后9～28 d(平均21.3 d)经静脉注入USPIO(0.3 ml/kg),分别于增强前、增强后24 h行MRI扫描,其中2只于48、72 h再行MRI扫描.扫描方案包括T1WI、FSET2WI、短时反转恢复(STIR)序列及梯度回波(GRE)T2*WI.测量USPIO增强前后滑膜的信噪比(SNR)及SNR变化(△SNR),行配对t检验,并与病理结果相对照.结果 9只大白兔右膝均成功诱导关节炎模型,病理显示滑膜增生,血管翳形成,有大量吞噬USPIO的滑膜巨噬细胞浸润;MRI示滑膜增厚,厚度为(2.07±0.97)mm;关节囊积液.注射USPIO后24 h,T1WI信号升高[△SNR为(41.91±27.94)%],T2WI和T2*WI信号下降[△SNR分别为(-34.92±11.77)%和(-57.24±16.05)%],各序列增强前后SNR差异有统计学意义(P＜0.05).注射后48 h,信号强度开始恢复,72 h信号基本同增强前.模型组左膝关节及假模型组双膝关节滑膜正常,关节囊无明显积液.结论 滑膜巨噬细胞吞噬氧化铁粒子是引起USPIO增强MRI信号改变的基础,GRE序列是反映USPIO增强效应的敏感序列。
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