Abstract
An ultrasensitive gene specific DNA sensor was developed by covalent immobilization of single stranded (ssDNA) probe on Au/PAMAM nanohybrid modified electrode for early detection of pathogen Streptococcus pyogenes infection in human. An electrochemical response was measured after hybridization with single stranded genomic DNA (ssG-DNA) of S. pyogenes from the patient samples. The nanohybrid sensor was characterized by FTIR, SEM and validated with patient real samples. The sensitivity of the sensor was 860.95 (μA/cm2)/ng DNA and lower limit of detection was 150fg in 6μL samples. The sensor is highly specific and can save damage of human mitral and aortic heart valves at early stage of infection by rapid diagnosis (30min) and medical care.
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