Abstract
Foodborne pathogens such as Clostridium perfringens can cause diverse illnesses and seriously threaten to human health, yet far less attention has been given to detecting these pathogenic bacteria. Herein, two morphologies of nanoceria were synthesized via adjusting the concentration of NaOH, and CeO2 nanorod has been utilized as sensing material to achieve sensitive and selective detection of C. perfringens DNA sequence due to its strong adsorption ability towards DNA compared to nanoparticle. The DNA probe was tightly immobilized on CeO2/chitosan modified electrode surface via metal coordination, and the DNA surface density was 2.51 × 10−10 mol/cm2. Under optimal experimental conditions, the electrochemical impedance biosensor displays favorable selectivity toward target DNA in comparison with base-mismatched and non-complementary DNA. The dynamic linear range of the proposed biosensor for detecting oligonucleotide sequence of Clostridium perfringens was from 1.0 × 10−14 to 1.0 × 10−7 mol/L. The detection limit was 7.06 × 10−15 mol/L. In comparison, differential pulse voltammetry (DPV) method quantified the target DNA with a detection limit of 1.95 × 10−15 mol/L. Moreover, the DNA biosensor could detect C. perfringens extracted DNA in dairy products and provided a potential application in food quality control.
Highlights
Foodborne pathogens that cause numerous illnesses have become a worldwide health problem [1].Clostridium perfringens is one of the most common foodborne pathogens
If CHIT is modified with CeO2 nanorods, it is possible to obtain novel functionalized materials that simultaneously have the properties of CHIT and CeO2 through incorporating their individual characteristics
CeO2 nanorods were synthesized via a simple hydrothermal method and used as sensing materials to detect Clostridium perfringens DNA sequence in dairy products
Summary
Foodborne pathogens that cause numerous illnesses have become a worldwide health problem [1]. Nanostructured CeO2 with controlled size, specific morphology, excellent conductivity and high surface charge is attracting much attention in the development of electrochemical DNA biosensor. If CHIT is modified with CeO2 nanorods, it is possible to obtain novel functionalized materials that simultaneously have the properties of CHIT (specific surface area and electrical conductive properties) and CeO2 (high DNA adsorption capacity) through incorporating their individual characteristics. There is no report on employing CeO2 /CHIT nanocomposite as sensing material to detect Clostridium perfringens. CeO2 nanorods were synthesized via a simple hydrothermal method and used as sensing materials to detect Clostridium perfringens DNA sequence in dairy products. CeO2 /CHIT nanocomposite was used for immobilizing the DNA probe on the electrode surface without employing any functional groups or intermediate linker. The rod-shaped CeO2 -based biosensor can be utilized as a potential sensing platform to detect foodborne pathogens effectively and conveniently.
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