Abstract

Forced by the progressive perfectionation of the ultramicrotomes and by the improvement of preparation methods, the ultrathin sectioning technique has gained high importance for the electron-microscopical studies of the fine structure of wood cell walls. Wood has its own problems for ultramicrotomy. During cutting, the inhomogeneity of the cell wall and its chemical nature result in the formation of wrinkles and holes in the ultrathin sections. These artefacts are not completely avoidable but can be reduced by suitable embedding and by choosing the most suitable knife to a minimum. It is pointed out that for the best and most reliable statement on the object the cutting of serial sections is indispensable.

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