Ultra-short laser structuring of 3D-microfabricated non-resorbable polystyrene polymer to anchor cellular adhesion

Abstract

Tissue engineering is emerging as a preferred choice for regeneration of irreversible bone tissue defects. It’s main “weapons” are the artificially created biocompatible three-dimensional cellular matrices that mimic the extracellular matrix’s structure and provide a mechanically stable inert substrate for the natural growth of cells. These porous scaffolds provide sufficient inner surface for cell adhesion, migration, proliferation and protrusion “inside” the temporal implant, facilitating in that way the natural bone ingrowth and vascularization. This leads, eventually, to overgrowing the outer and inner surface of the biomimetic implant with recipient self-formed bone tissue, thus avoiding a possible immune response.In the current study 3D Polystyrene (PS) non-resorbable synthetic cellular bone scaffolds, produced by 3D microfabrication technology, were additionally structured by applying femtosecond (fs) laser radiation with variable parameters thus achieving optimal laser processing conditions, for creation of 3D matrix with enhanced fiber surface properties. The main aim is the establishment of surface structured microporous biomimetic scaffolds, providing improved cell adhesion, infiltration and formation of extracellular matrix. The 3D laser functionalized PS samples were investigated by Scanning Electron Microscopy (SEM), Energy Dispersive X-Ray analysis (EDX), confocal (optical profilometer) and roughness analyses for evaluation of their morphological and chemical properties before and after fs structuring was performed. Water contact angle (WCA) evaluation was also conducted. Future experimental work will include in vitro cellular studies for determining the optimal structuring laser parameters, providing osteoconductive and osteoinductive qualities of the 3D fs-microstructured PS scaffolds.