Abstract
Streptococcus suis (S. suis) can decrease its virulence or modify local conditions through biofilm formation, which promotes infection persistence in vivo. Biofilm formation is an important cause of chronic drug-resistant S. suis infection. The aim of this study was to evaluate whether tylosin effectively inhibits S. suis biofilm formation by interacting with O-acetylserine (thiol)-lyase B (CysM), a key enzymatic regulator of cysteine synthesis. Biofilm formation of the mutant (ΔcysM) strain was significantly lower compared to the wild-type ATCC 700794 strain. Tylosin inhibited cysM gene expression, decreased extracellular matrix contents, and reduced cysteine, homocysteine, and S-adenosylmethionine levels, indicating its potential value as an effective inhibitor of S. suis biofilm formation. Furthermore, using biolayer interferometry technology and fourier-transform infrared spectroscopy, we found that tylosin and CysM could be combined directly. Overall, these results provide evidence that tylosin inhibits S. suis biofilm formation by interacting with CysM.
Highlights
Streptococcus suis (S. suis) can cause meningitis, septicemia, pneumonia, endocarditis, and arthritis in pigs
We previously found that 1/4 minimum inhibitory concentration (MIC) of tylosin effectively inhibits S. suis biofilm formation [27]
The ability of S. suis to form a biofilm plays an important role in its virulence and the development of drug resistance [16]
Summary
Streptococcus suis (S. suis) can cause meningitis, septicemia, pneumonia, endocarditis, and arthritis in pigs. Because it is a zoonotic pathogen, it poses a significant harm to public safety [1]. Most carrier strains are non-virulent, pathogenic strains can colonize the respiratory mucosal surfaces without causing clinical disease, which is the first step in the development of invasive disease in pigs, with subsequent hematogenous and/or lymphogenous dissemination [4]. The persistence of S. suis in the oral cavity can contribute to increased disease pathogenicity [5]. S. suis biofilm formation establishes important conditions to ensure its long-term persistence, and Tylosin Inhibits S. suis Biofilm Formation decrease its virulence in order to establish long-term infections such as meningitis and endocarditis in its host
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
