Abstract
Two-dimensional double quantum 1H NMR spectra are recorded for a protein. The application of this technique to macromolecules is shown not to be impeded by the long preparation pulse sequence essential for this experiment. The identification of spin systems by analysis of the double quantum spectrum is illustrated. Since double quantum spectra do not contain diagonal peaks, connectivities between almost degenerate signals can be detected. By analysis of remote connectivities (i) it can be established whether amide and β-protons belong to the same spin system or not, (ii) degeneracy of β-proton chemical shifts can be demonstrated, and (iii) glycine amide protons can be distinguished from all others.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochemical and Biophysical Research Communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
